TY - CHAP. T1 - Isolation and culture of adult mouse cardiac myocytes. AU - O'Connell, Timothy D. AU - Rodrigo, Manoj C. AU - Simpson, Paul C. PY - 2007/2/5Cited by: 266. May 21, 2014 · Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy. Blebbistatin extends culture life of adult mouse cardiac myocytes and allows efficient and stable transgene expression. Am. J. Physiol. Heart Circ. Physiol. 2008; 294:1667–1674. Lou Q, Li W, Efimov IR. The role of dynamic instability Cited by: 23.
Feb 13, 2001 · Dual modulation of cell survival and cell death by β 2-adrenergic signaling in adult mouse cardiac myocytes. In summary, stimulation of β 2-AR concurrently activates cardiac survival and apoptotic signals in adult mouse cardiac myocytes, with the survival signal predominating.Cited by: 492. However, studies at the cellular level have been hampered by a lack of suitable techniques for isolating and culturing calcium-tolerant, adult mouse cardiac myocytes. We have developed a straightforward, reproducible protocol for isolating and culturing large numbers of adult mouse cardiac myocytes.Cited by: 266.
B lymphocytes and cardiac myocytes from the mouse. The basis for the selection of these two cell types is described elsewhere(1,2), as is the description of the cell culture system for mouse B lymphocytes (3). This manuscript describes the cell culture system for adult mouse cardiac myocytes. Cardiac myocytes respond to a variety of hormonal,Cited by: 31. The Alliance for Cellular Signaling (AfCS) has chosen cultured adult mouse cardiac myocytes as a system for study- ing cellular signaling. We developed a reproducible proto- col for the isolation.
Feb 13, 2001 · One potential mechanism is that cardiac β2-AR can activate both Gs and Gi proteins, whereas cardiac β1-AR couples only to Gs. To avoid complicated crosstalk between β-AR subtypes, we expressed β1-AR or β2-AR individually in adult β1/β2-AR double knockout mouse cardiac myocytes by using adenoviral gene transfer.Cited by: 492. Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy. Daxiang Li 1, Jian Wu 1, Simpon, P. C. Isolation and culture of adult mouse cardiac myocytes. Methods in Molecular Biology. Vivanco, F. 35, Humana Press. 271-296 (2007). Nilolskaya, A., Sharma, V. Cell culture models and methods.Cited by: 23.